Last data update: May 20, 2024. (Total: 46824 publications since 2009)
Records 1-2 (of 2 Records) |
Query Trace: Anyanwu J[original query] |
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An Mpox-related death in the United States
Alarcón J , Kim M , Terashita D , Davar K , Garrigues JM , Guccione JP , Evans MG , Hemarajata P , Wald-Dickler N , Holtom P , Garcia Tome R , Anyanwu L , Shah NK , Miller M , Smith T , Matheny A , Davidson W , Hutson CL , Lucas J , Ukpo OC , Green NM , Balter SE . N Engl J Med 2023 388 (13) 1246-1247 Since May 2022, when the multinational mpox (formerly known as monkeypox) clade IIb virus outbreak was first reported, more than 30,000 cases have been identified in the United States.1 In one study involving more than 1900 patients with mpox, more than 35% of the patients also had human immunodeficiency virus (HIV) infection.2 | | We report a death due to mpox in a patient in the United States. A 33-year-old man with HIV infection (CD4+ T-cell count, <35 per cubic millimeter) and recently treated syphilis became infected with mpox virus (MPXV) (clade IIb). He received two courses of oral tecovirimat (from Aug. 6 through Aug. 20, 2022, and from Aug. 21 through Sept. 4, 2022) and died on hospital day 27. |
Dried tube specimens: a simple and cost-effective method for preparation of HIV proficiency testing panels and quality control materials for use in resource-limited settings
Parekh BS , Anyanwu J , Patel H , Downer M , Kalou M , Gichimu C , Keipkerich BS , Clement N , Omondi M , Mayer O , Ou CY , Nkengasong JN . J Virol Methods 2009 163 (2) 295-300 HIV testing has rapidly expanded worldwide, but proficiency testing (PT) programs to monitor and improve the quality of testing are often lacking in resource-limited settings (RLS). Traditional PT programs and quality control reagents use serum/plasma specimens requiring stringent conditions for storage and transportation. A novel, simple and easy to use approach, based on dried tube specimens (DTS), was developed that can help monitor the quality of HIV antibody testing in RLS. DTS were prepared by drying 20mul of specimen overnight at room temperature. The addition of a green dye (0.1%) made the DTS pellets visible without affecting the test results. Before testing, the DTS were rehydrated with 200mul of PBS/Tween buffer. A panel of 303 DTS samples (135 HIV positive and 168 negative) was evaluated with two rapid tests. Sensitivity and specificity with the Determine HIV-1/2 test were 99.3% and 99.4%, respectively, and with OraQuick were 98.5% and 100%, respectively. Stability studies showed that HIV-specific antibodies in the DTS specimens were stable at 4 degrees and 25 degrees for 4 weeks, with only marginal decline at 37 degrees and 45 degrees C over 4 weeks. The DTS-based PT program was piloted successfully in 24 testing sites in Kenya. Results demonstrate that the DTS is a simple to use, practical method to prepare and distribute PT panels and quality control specimens to monitor HIV testing practices in RLS. |
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